@article {20886, title = {A molecular phylogeny of Eumorpha (Lepidoptera: Sphingidae) and the evolution of anti-predator larval eyespots}, journal = {Systematic Entomology}, volume = {40}, year = {2015}, pages = {401-408}, chapter = {401}, keywords = {BAYESIAN INFERENCE, BEHAVIOUR, BIOGEOGRAPHY, CAD, COI, DEFENCE, DNA, EF-1ALPHA, EUMORPHA, EYESPOT, IMMATURE STAGES, MAXIMUM LIKELIHOOD, MIMICRY, PHYLOGENY, RASP, RAXML, SPHINGIDAE, WINGLESS, WORLDMAP1}, doi = {10.1111/syen.12111}, url = {https://doi.org/10.1111/syen.12111}, author = {Ponce, F.V. and Breinholt, J.W. and Hossie, T. and Barber, J.R. and Janzen, D.H. and Hallwachs, W. and Kawahara, A.Y.} } @article {20549, title = {When species matches are unavailable are DNA barcodes correctly assigned to higher taxa? An assessment using sphingid moths}, journal = {BMC Ecology}, volume = {11}, year = {2011}, month = {2011}, pages = {18}, chapter = {18}, abstract = {

\"Background

When a specimen belongs to a species not yet represented in DNA barcode reference libraries there is disagreement over the effectiveness of using sequence comparisons to assign the query accurately to a higher taxon. Library completeness and the assignment criteria used have been proposed as critical factors affecting the accuracy of such assignments but have not been thoroughly investigated. We explored the accuracy of assignments to genus, tribe and subfamily in the Sphingidae, using the almost complete global DNA barcode reference library (1095 species) available for this family. Costa Rican sphingids (118 species), a well-documented, diverse subset of the family, with each of the tribes and subfamilies represented were used as queries. We simulated libraries with different levels of completeness (10-100\% of the available species), and recorded assignments (positive or ambiguous) and their accuracy (true or false) under six criteria.

Results

A liberal tree-based criterion assigned 83\% of queries accurately to genus, 74\% to tribe and 90\% to subfamily, compared to a strict tree-based criterion, which assigned 75\% of queries accurately to genus, 66\% to tribe and 84\% to subfamily, with a library containing 100\% of available species (but excluding the species of the query). The greater number of true positives delivered by more relaxed criteria was negatively balanced by the occurrence of more false positives. This effect was most sharply observed with libraries of the lowest completeness where, for example at the genus level, 32\% of assignments were false positives with the liberal criterion versus \< 1\% when using the strict. We observed little difference (\< 8\% using the liberal criterion) however, in the overall accuracy of the assignments between the lowest and highest levels of library completeness at the tribe and subfamily level.

Conclusions

Our results suggest that when using a strict tree-based criterion for higher taxon assignment with DNA barcodes, the likelihood of assigning a query a genus name incorrectly is very low, if a genus name is provided it has a high likelihood of being accurate, and if no genus match is available the query can nevertheless be assigned to a subfamily with high accuracy regardless of library completeness. DNA barcoding often correctly assigned sphingid moths to higher taxa when species matches were unavailable, suggesting that barcode reference libraries can be useful for higher taxon assignments long before they achieve complete species coverage.\"

}, keywords = {BARCODING, DNA, HIGHER CLASSIFICATION, IDENTIFICATION, SPHINGIDAE}, url = {http://www.biomedcentral.com/1472-6785/11/18}, author = {Wilson, J.J. and Rougerie, R. and Schonfeld, J. and Janzen, D.H. and Hallwachs, W. and Hajibabaei, M. and Kitching, I.J. and Haxaire, J. and Hebert, P.D.N.} } @article {18607, title = {Area de Conservaci{\'o}n Guanacaste (ACG), northwestern Costa Rica. Caterpillars, pupae, butterflies \& moths}, year = {2018}, month = {2005}, keywords = {APATELODIDAE, CATE DIAGNOSIS, COSTA RICA, FOODPLANT, IMMATURE MORPHOLOGY, IMMATURE STAGES, LASIOCAMPIDAE, SATURNIIDAE, SPHINGIDAE}, url = {http://janzen.sas.upenn.edu/caterpillars/database.lasso}, author = {Janzen, D.H. and Hallwachs, W.} } @article {18606, title = {Wedding biodiversity inventory of a large and complex Lepidoptera fauna with DNA barcoding}, journal = {Philosophical Transactions of the Royal Society of London}, volume = {360}, year = {2005}, note = {B}, month = {2005}, pages = {1835-1845}, keywords = {BARCODING, BIODIVERSITY, COI, COSTA RICA, CRYPTIC SPECIES, DNA, HESPERIIDAE, SATURNIIDAE, SPHINGIDAE, TAXONOMY}, doi = {10.1098/rstb.2005.1715}, author = {Janzen, D.H. and Hajibabaei, M. and Burns, J.M. and Hallwachs, W. and Remigio, E. and Hebert, P.D.N.} } @article {18508, title = {An eyespot that "blinks": an open and shut case of eye mimicry in Eumorpha caterpillars (Lepidoptera: Sphingidae)}, journal = {Journal of Natural History}, volume = {47}, year = {2013}, note = {http://dx.doi.org/10.1080/00222933.2013.791935; ; ;}, month = {2013}, pages = {2915-2926}, chapter = {2915}, keywords = {COSTA RICA, EUMORPHA, EYESPOT, FOODPLANT, IMMATURE STAGES, MIMICRY, SPHINGIDAE, WORLDMAP1}, author = {Hossie, T.J. and Sherratt, T.N. and Janzen, D.H. and Hallwachs, W.} } @article {18201, title = {DNA barcodes distinguish species of tropical Lepidoptera}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {103}, year = {2006}, month = {01/2006}, pages = {968-971}, chapter = {968}, keywords = {BARCODING, CLASSIFICATION, COI, COSTA RICA, HESPERIIDAE, LEPIDOPTERA, SATURNIIDAE, SPHINGIDAE}, url = {https://www.pnas.org/doi/epdf/10.1073/pnas.0510466103}, author = {Hajibabaei, M. and Janzen, D.H. and Burns, J.M. and Hallwachs, W. and Hebert, P.D.N.} }