02011nas a2200205   4500008004100000245016700041210006900208260001200277300000800289490000700297520136500304653001401669653000801683653000801691653001201699653002501711100001401736700001101750856004401761       2022                        eng d00aExploring large-scale patterns of genetic variation in the COI gene among Insecta: implications for DNA barcoding and threshold-based species delimitation studies0 aExploring largescale patterns of genetic variation in the COI ge  c04/2022  a4250 v133 a<p>&quot;The genetic variation in the COI gene has had a great effect on the final results of species delimitation studies. However, little research has comprehensively investigated the genetic divergence in COI among Insecta. The fast-growing COI data in BOLD provide an opportunity for the comprehensive appraisal of the genetic variation in COI among Insecta. We calculated the K2P distance of 64,414 insect species downloaded from BOLD. The match ratios of the clustering analysis, based on different thresholds, were also compared among 4288 genera (35,068 species). The results indicate that approximately one-quarter of the species of Insecta showed high intraspecific genetic variation (&gt;3%), and a conservative estimate of this proportion ranges from 12.05% to 22.58%. The application of empirical thresholds (e.g., 2% and 3%) in the clustering analysis may result in the overestimation of the species diversity. If the minimum interspecific genetic distance of the congeneric species is greater than or equal to 2%, it is possible to avoid overestimating the species diversity on the basis of the empirical thresholds. In comparison to the fixed thresholds, the &ldquo;threshOpt&rdquo; and &ldquo;localMinima&rdquo; algorithms are recommended for the provision of a reference threshold for threshold-based species delimitation studies.&quot;</p>
10aBARCODING10aCOI10aDNA10aINSECTA10aSPECIES DELIMITATION1 aZhang, H.1 aBu, W.  uhttps://doi.org/10.3390/insects13050425