|Publication Type:||Journal Article|
|Year of Publication:||2005|
|Authors:||A. K. Hundsdoerfer, Wink M.|
|Journal:||Zeitschrift für Naturforschung|
|Keywords:||CRAMBIDAE, DNA, ISSR, PIERIDAE, SPHINGIDAE|
"The variability level of the ISSR (inter-simple sequences repeat) primer (GACA)4 was examined in the three Lepidoptera families Pyralidae, Sphingidae and Pieridae. Our study shows that the tetra-repeat (GACA)n is evidently present in sufficient numbers in these butterflies to provide informative DNA fingerprints. The variability is mostly rather high, but within a comparable range to other ISSR studies. Although less polymorphisms may be encountered in some butterfly families, this study indicates that high variability of this marker may be a common characteristic of Lepidoptera genomes. An appeal for a minimal level of standardization of ISSR-PCR data analysis is formulated to enable an exact comparison between the groups of organisms studied with this fingerprint technique."
New source of genetic polymorphisms in Lepidoptera?