<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Markee, A.</style></author><author><style face="normal" font="default" size="100%">Godfrey, R.K.</style></author><author><style face="normal" font="default" size="100%">Frandsen, P.B.</style></author><author><style face="normal" font="default" size="100%">Weng, Y.-M.</style></author><author><style face="normal" font="default" size="100%">Triant, D.A.</style></author><author><style face="normal" font="default" size="100%">Kawahara, A.Y.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">De novo long-read genome assembly and annotation of the Luna Moth (Actias luna) fully resolves repeat-rich silk genes</style></title><secondary-title><style face="normal" font="default" size="100%">Genome Biology and Evolution</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">ACTIAS</style></keyword><keyword><style  face="normal" font="default" size="100%">FIBROIN</style></keyword><keyword><style  face="normal" font="default" size="100%">GENOME ASSEMBLY</style></keyword><keyword><style  face="normal" font="default" size="100%">PACBIO LONG READS</style></keyword><keyword><style  face="normal" font="default" size="100%">SATURNIIDAE</style></keyword><keyword><style  face="normal" font="default" size="100%">SILK</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year><pub-dates><date><style  face="normal" font="default" size="100%">3/7/2024</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">https://doi.org/10.1093/gbe/evae148</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">16</style></volume><pages><style face="normal" font="default" size="100%">evae148</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&amp;quot;We present the first long-read de novo assembly and annotation of the luna moth (&lt;em&gt;Actias luna&lt;/em&gt;) and provide the full characterization of heavy chain fibroin (h-fibroin), a long and highly repetitive gene (&amp;gt;20 kb) essential in silk fiber production. There are &amp;gt;160,000 described species of moths and butterflies (Lepidoptera), but only within the last 5 years have we begun to recover high-quality annotated whole genomes across the order that capture h-fibroin. Using PacBio HiFi reads, we produce the first high-quality long-read reference genome for this species. The assembled genome has a length of 532 Mb, a contig N50 of 16.8 Mb, an L50 of 14 contigs, and 99.4% completeness (BUSCO). Our annotation using &lt;em&gt;Bombyx mori&lt;/em&gt; protein and &lt;em&gt;A. luna&lt;/em&gt; RNAseq evidence captured a total of 20,866 genes at 98.9% completeness with 10,267 functionally annotated proteins and a full-length h-fibroin annotation of 2,679 amino acid residues.&amp;quot;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">7</style></issue><work-type><style face="normal" font="default" size="100%">Advance access</style></work-type><section><style face="normal" font="default" size="100%">evae148</style></section></record></records></xml>