<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vanitha, C.</style></author><author><style face="normal" font="default" size="100%">Balakrishnan, I.K.</style></author><author><style face="normal" font="default" size="100%">Debnath, R.</style></author><author><style face="normal" font="default" size="100%">Lavanya, C.</style></author><author><style face="normal" font="default" size="100%">Tulsi_Naik, K.S.</style></author><author><style face="normal" font="default" size="100%">Moorthy, S.M.</style></author><author><style face="normal" font="default" size="100%">Ramesh, K.V.</style></author><author><style face="normal" font="default" size="100%">Dubey, H.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genome-wide in-silico identification of microsatellites in Eri Silkworm, Samia ricini (Lepidoptera: Saturniidae)</style></title><secondary-title><style face="normal" font="default" size="100%">Ecology, Environment &amp; Conservation</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">GENE ONTOLOGY</style></keyword><keyword><style  face="normal" font="default" size="100%">ISSR</style></keyword><keyword><style  face="normal" font="default" size="100%">MICROSATELLITES</style></keyword><keyword><style  face="normal" font="default" size="100%">SAMIA</style></keyword><keyword><style  face="normal" font="default" size="100%">SATURNIIDAE</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2024</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://doi.org/10.53550/EEC.2024.v30i04.070</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">30</style></volume><pages><style face="normal" font="default" size="100%">1888-1898</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;&amp;quot;Effective utilization of microsatellite or simple sequence repeat (SSR) markers along with ongoing advanced technologies in the field of molecular biology have made great advantages. In the present study, the genome-wide distribution of SSRs in Eri silkworm, &lt;em&gt;Samia ricini &lt;/em&gt;Donovan was studied along with the &lt;em&gt;in-silico &lt;/em&gt;transferability of SSR primers to the closely related species &lt;em&gt;S. wangi&lt;/em&gt; and &lt;em&gt;S. watsoni&lt;/em&gt;. We also analyzed and compared the SSRs in two closely related &lt;em&gt;Samia &lt;/em&gt;species with the motifs identified in &lt;em&gt;S. ricini&lt;/em&gt; genome. The overall abundance of SSR motifs was more in &lt;em&gt;S. watsoni &lt;/em&gt;compared to &lt;em&gt;S. ricini&lt;/em&gt; and &lt;em&gt;S. wangi&lt;/em&gt;. The cross-amplification analysis through&lt;em&gt; in-silico&lt;/em&gt; PCR method with the 25,252 SSR primer pairs developed for S. ricini, showed that 2,978 and 1,508 primer sets were amplified in the S. wangi and in S. watsoni, respectively. The distribution of SSR motifs in S. ricini genome showed a higher proportion of SSR motifs in intergenic regions than exonic and intronic regions (41%, 30% and 29%, respectively). The gene ontology analysis of the SSR containing genes showed that a greater number of genes were associated with the biological process (889) followed by molecular function (215) and cellular components (213). The SSRs present in the coding region revealed, Alanine (Ala) as the most abundant amino acid in these loci followed by Tyrosine (Tyr) and Methionine (Met) in the coding region of the &lt;em&gt;Samia ricini&lt;/em&gt;. To validate our&lt;em&gt; in-silico&lt;/em&gt; analysis, we randomly selected 20 SSR primers and amplified them in different morphotypes of Eri silkworm.&amp;quot;&lt;/p&gt;
</style></abstract><issue><style face="normal" font="default" size="100%">4</style></issue><section><style face="normal" font="default" size="100%">1888</style></section></record></records></xml>