02544nas a2200277 4500008004100000245011200041210006900153300001000222490000700232520175300239653001501992653002602007653001502033653001202048653001202060653002102072653001502093653001502108100001702123700001602140700001502156700001602171700001602187700001402203856004902217 2012 eng d00aAdipokinetic hormones (AKHs) of sphingid Lepidoptera, including the identification of a second M. sexta AKH0 aAdipokinetic hormones AKHs of sphingid Lepidoptera including the a44-500 v343 a

"The adipokinetic hormones (AKHs) from the corpora cardiaca (CC) of representative species from all three subfamilies of the Sphingidae (hawkmoths) were investigated using matrix-assisted laser desorption–ionization time-of-flight (MALDI-TOF) and liquid chromatography electrospray ion trap mass spectrometry (LC–ESI MS), including a re-examination of the AKH complement of the tobacco hawkmoth, Manduca sexta. In addition to larvae and adults of M. sexta (subfamily: Sphinginae), adults from the following subfamilies were examined: Macroglossinae (large elephant hawkmoth, Deilephila elpenor), Smerinthinae (poplar hawkmoth, Laothoe populi and eyed hawkmoth, Smerinthus ocellata), and Sphinginae (death's head hawkmoth, Acherontia atropos). All moths are shown to have the nonapeptide Manse-AKH (pELTFTSSGWamide) in their CC, together with a second AKH, which, on the basis of mass ions ([M+Na]+, [M+K]+) and partial sequence analysis is identical in all species examined. The structure of this AKH was elucidated from peptides leached out of the CC of adult M. sexta and shown, by ESI-collision-induced dissociation (CID) tandem mass spectrometry (MS/MS), to be a novel decapeptide AKH with a sequence of pELTFSSGWGQamide. The new peptide has been code named Manse-AKH-II. Sequence confirmation was obtained from identical MS studies with synthetic Manse-AKH-II and with the native peptide. Manse-AKH-II has significant lipid-mobilizing activity when injected at low dose (5 pmol) into newly emerged adult M. sexta. The potential implications of a second AKH, in M. sexta in particular, are discussed in relation to putative receptor(s)."

10aACHERONTIA10aADIPOKINETIC HORMONES10aDEILEPHILA10aLAOTHOE10aMANDUCA10aMASS SPECTOMETRY10aSMERINTHUS10aSPHINGIDAE1 aWeaver, R.J.1 aMarco, H.G.1 aŠimek, P.1 aAudsley, N.1 aClark, K.D.1 aGäde, G. uhttps://sphingidae.myspecies.info/node/22314