03262nas a2200349 4500008004100000245010600041210006900147260001200216300001100228490000600239520232700245653002202572653001702594653001202611653001402623653001402637653001502651653001602666653002702682653001502709653001502724653001102739653000802750100001302758700001102771700001802782700001802800700001802818700001502836700001202851856004902863 2021 eng d00aVariation in Manduca sexta pollination-related floral traits and reproduction in a wild tobacco plant0 aVariation in Manduca sexta pollinationrelated floral traits and  c07/2021 a6804630 v93 a

"Most flowering plants depend on animal pollination for successful sexual reproduction. Floral signals such as color, shape, and odor are crucial in establishing this (often mutualistic) interaction. Plant and pollinator phenotypes can vary temporally but also spatially, thus creating mosaic-like patterns of local adaptations. Here, we investigated natural variation in floral morphology, flower volatile emission, and phenology in four accessions of a self-compatible wild tobacco, Nicotiana attenuata, to assess how these traits match the sensory perception of a known pollinator, the hawkmoth Manduca sexta. These accessions differ in floral traits and also in their habitat altitudes. Based on habitat temperatures, the accession occurring at the highest altitude (California) is less likely to be visited by M. sexta, while the others (Arizona, Utah 1, and Utah 2) are known to receive M. sexta pollinations. The accessions varied significantly in flower morphologies, volatile emissions, flower opening, and phenology, traits likely important for M. sexta perception and floral handling. In wind tunnel assays, we assessed the seed set of emasculated flowers after M. sexta visitation and of natural selfed and hand-pollinated selfed flowers. After moth visitations, plants of two accessions (Arizona and Utah 2) produced more capsules than the other two, consistent with predictions that accessions co-occurring with M. sexta would benefit more from the pollination services of this moth. We quantified flower and capsule production in four accessions in a glasshouse assay without pollinators to assess the potential for self-pollination. The two Utah accessions set significantly more seeds after pollen supplementation compared with those of autonomous selfing flowers, suggesting a greater opportunistic benefit from efficient pollinators than the other two. Moreover, emasculated flowers of the accession with the most exposed stigma (Utah 2) produced the greatest seed set after M. sexta visitation. This study reveals intraspecific variation in pollination syndromes that illuminate the potential of a plant species to adapt to local pollinator communities, changing environments, and altered pollination networks."

10aFLORAL MORPHOLOGY10aFLORAL SCENT10aMANDUCA10aNICOTIANA10aPHENOLOGY10aPHYSIOLOGY10aPOLLINATION10aPOLLINATOR SPECIFICITY10aSOLANACEAE10aSPHINGIDAE10aTRAITS10aUSA1 aBing, J.1 aLi, X.1 aHaverkamp, A.1 aBaldwin, I.T.1 aHansson, B.S.1 aKnaden, M.1 aYon, F. uhttps://sphingidae.myspecies.info/node/22349