Sphingidae Taxonomic Inventory

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Exploring large-scale patterns of genetic variation in the COI gene among Insecta: implications for DNA barcoding and threshold-based species delimitation studies

Publication Type:Journal Article
Year of Publication:2022
Authors:H. Zhang, Bu W.
Journal:Insects
Volume:13
Start Page:425
Pagination:425
Date Published:04/2022
Keywords:BARCODING, COI, DNA, INSECTA, SPECIES DELIMITATION
Abstract:

"The genetic variation in the COI gene has had a great effect on the final results of species delimitation studies. However, little research has comprehensively investigated the genetic divergence in COI among Insecta. The fast-growing COI data in BOLD provide an opportunity for the comprehensive appraisal of the genetic variation in COI among Insecta. We calculated the K2P distance of 64,414 insect species downloaded from BOLD. The match ratios of the clustering analysis, based on different thresholds, were also compared among 4288 genera (35,068 species). The results indicate that approximately one-quarter of the species of Insecta showed high intraspecific genetic variation (>3%), and a conservative estimate of this proportion ranges from 12.05% to 22.58%. The application of empirical thresholds (e.g., 2% and 3%) in the clustering analysis may result in the overestimation of the species diversity. If the minimum interspecific genetic distance of the congeneric species is greater than or equal to 2%, it is possible to avoid overestimating the species diversity on the basis of the empirical thresholds. In comparison to the fixed thresholds, the “threshOpt” and “localMinima” algorithms are recommended for the provision of a reference threshold for threshold-based species delimitation studies."

URL:https://doi.org/10.3390/insects13050425
DOI:10.3390/insects13050425
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith